Measurements of inner mucilage and seed width for Arabidopsis natural accessions - dataset 3 (ICPSR doi:10.15454/LBUN4X)

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Part 1: Document Description
Part 2: Study Description
Part 3: Data Files Description
Part 4: Variable Description
Entire Codebook

Document Description

Citation

Title:

Measurements of inner mucilage and seed width for Arabidopsis natural accessions - dataset 3

Identification Number:

doi:10.15454/LBUN4X

Distributor:

Portail Data INRAE

Date of Distribution:

2021-02-14

Version:

1

Bibliographic Citation:

Berger, Adeline; Sallé, Christine; North, Helen M., 2021, "Measurements of inner mucilage and seed width for Arabidopsis natural accessions - dataset 3", https://doi.org/10.15454/LBUN4X, Portail Data INRAE, V1, UNF:6:8oAFPY5mhQPk93K1BScRBQ== [fileUNF]

Study Description

Citation

Title:

Measurements of inner mucilage and seed width for Arabidopsis natural accessions - dataset 3

Identification Number:

doi:10.15454/LBUN4X

Authoring Entity:

Berger, Adeline (INRA - Institut National de la Recherche Agronomique)

Sallé, Christine (INRA - Institut National de la Recherche Agronomique)

North, Helen M. (INRA - Institut National de la Recherche Agronomique)

Grant Number:

ANR-14-CE19-0001

Distributor:

Portail Data INRAE

Access Authority:

North, Helen M.

Depositor:

North, Helen

Date of Deposit:

2020-05-05

Study Scope

Keywords:

Biodiversity and Ecology, Mucilage, Seed, Arabidopsis

Abstract:

The dataset comprises values of individual measurements of the two traits seed width and mucilage and seed width for 19 accessions having atypical outer mucilage properties (Fabrissin et al., 2019). Accessions are from the Versailles Arabidopsis stock center (http://publiclines.inra.fr/naturalAccession/index) and are listed by their Versailles identification number in a four-digit format. Seed lots were produced from plants in two cultivations series c or d and each lot is a bulk of seed from 10 - 12 individual plants. Measurements were obtained following immunolabelling and staining of seeds with an anti-RG-I antibody (INRA-RU1) and the cellulose specific fluorescent dye Direct Red 23 (DR23). As outer mucilage is lost during the immunolabelling procedure seeds analysed correspond to sample type 5 (inner mucilage and seed (hydrated). Observations was performed with a Zeiss LSM710 confocal microscope using 488nm or 561nm lasers to excite Alexa Fluor 488® or DR23, respectively. Fluorescence emission was detected between 500 and 550nm for Alexa fluor 488® and 565 and 640nm for DR23. For each seed lot, measurements were obtained from 30 seeds using Zen software. The dataset comprises 4560 values corresponding to measurements of 30 seeds from a seed lot.

Kind of Data:

Dataset

Methodology and Processing

Sources Statement

Data Access

Notes:

<img src="https://www.etalab.gouv.fr/wp-content/uploads/2011/10/licence-ouverte-open-licence.gif" alt="Licence Ouverte" height="100"><a href="https://www.etalab.gouv.fr/licence-ouverte-open-licence">Licence Ouverte / Open Licence Version 2.0</a> compatible CC BY

Other Study Description Materials

Other Reference Note(s)

Poulain D, Botran L, North HM, Ralet M-C (2019) Composition and physicochemical properties of outer mucilage from seeds of Arabidopsis natural accessions. AoB PLANTS 11: plz031

Fabrissin I, Cueff G, Berger A, Granier F, Sallé C, Poulain D, Ralet M-C, North HM. (2019) Natural variation reveals a key role for rhamnogalacturonan I in seed outer mucilage and pinpoints underlying genes. Plant Physiol. 181: 1498-1518.

File Description--f104431

File: dataset_3.tab

  • Number of cases: 4560

  • No. of variables per record: 10

  • Type of File: text/tab-separated-values

Notes:

UNF:6:8oAFPY5mhQPk93K1BScRBQ==

Variable Description

List of Variables:

Variables

samplecode

f104431 Location:

Variable Format: character

Notes: UNF:6:7xlga9Svyrehg3sVyxFniA==

accession

f104431 Location:

Summary Statistics: Mean 275.73684210526307; StDev 144.00220883926352; Min. 13.0; Valid 4560.0; Max. 549.0

Variable Format: numeric

Notes: UNF:6:5fLOdWwhJD4tGZlBX6p8tQ==

cultivationseries

f104431 Location:

Variable Format: character

Notes: UNF:6:HT2jdcudmXgDLjsARjECsA==

seedlot

f104431 Location:

Summary Statistics: StDev 0.5000548335802493; Max. 2.0; Mean 1.5; Valid 4560.0; Min. 1.0

Variable Format: numeric

Notes: UNF:6:4ZgeLmZfhtdWrELHj3NqSw==

sampletype

f104431 Location:

Summary Statistics: Valid 4560.0; Max. 5.0; Min. 5.0; StDev 0.0; Mean 5.0

Variable Format: numeric

Notes: UNF:6:WnrJdyd0bimZBYy4XZvXJg==

Seedmeasured

f104431 Location:

Summary Statistics: Mean 15.5; Min. 1.0; Valid 4560.0; StDev 8.656390666085699; Max. 30.0

Variable Format: numeric

Notes: UNF:6:mmSy5f+lVA2elFV2GB3a9w==

variable

f104431 Location:

Variable Format: character

Notes: UNF:6:9CGm3Xowwgh3CG5/91/oAg==

value

f104431 Location:

Summary Statistics: Max. 833.66; Valid 4560.0; Min. 205.95; StDev 134.55757861714355; Mean 453.833608114035

Variable Format: numeric

Notes: UNF:6:lMJs1ASfB8Vqim+T0Il7zg==

date

f104431 Location:

Summary Statistics: StDev NaN; Min. NaN; Valid 0.0; Mean NaN; Max. NaN;

Variable Format: numeric

Notes: UNF:6:UVcxDZOY1AfTJRXZ8TiqpQ==

time

f104431 Location:

Summary Statistics: Valid 0.0; Min. NaN; StDev NaN; Max. NaN; Mean NaN

Variable Format: numeric

Notes: UNF:6:UVcxDZOY1AfTJRXZ8TiqpQ==