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Nov 15, 2019
Zemb, Olivier, 2019, "Absolute quantitation of microbes using 16S rRNA gene metabarcoding: a rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike-in standard", https://doi.org/10.15454/YWMMH4, Portail Data INRAE, V1, UNF:6:F0zGeneCWQ7Kv1ouvU+bEQ== [fileUNF]
Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial communities, by estimating the relative abundance of microbes. Here we present a method to retrieve the concentrations of the 16S rRNA gene per gram of any environmental sample using a synthetic standar...
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